Author Archives: admin

Home / Articles posted by admin

New PhD opportunity

PhD opportunity – Development and application of a multi-component 3-D in vitro model for
predictive pharmacokinetics of environmental pharmaceuticals in fish.
In collaboration with Prof Christer Hogstrand and Dr Leon Barron (King’s College London) and Dr
Stewart Owen (AstraZeneca)

http://lido-dtp.ac.uk/hogstrand.html

Cocaine found in Suffolk Shrimps

Our recent study identified the presence of cocaine and
other drugs of abuse in aquatic shrimps throughout rivers in Suffolk, which received widespread
international media attention (https://www.bbc.co.uk/news/uk-england-suffolk-48117678). Read
more at Miller, T.H., Ng, K.T., Bury, S.T., Bury, S.E., Bury, N.R., Barron, L.P. 2019 Biomonitoring of
pesticides, pharmaceuticals and illicit drugs in a freshwater invertebrate to estimate toxic or effect
pressure. Environment International.

Awards for Justine Oakes and Nic Bury

Awards at the Sustainable Ecological Engineering Design for Society Conference (SEEDS), September
2019 for Justine Oakes and Nic Bury of the Suffolk Sustainability Institute

Dr Elisabeth Chang has successful defended her thesis

Dr Elisabeth Chang – Elisabeth has successful defended her thesis entiteled “ Understadning movement of pharmaceuticals across rainbow trout gills using an in vitro gill cell culture”

Dr Anna Lavelle has successfully defended her thesis

Dr Anna Lavelle – Anna has successfully defended her thesis entitled “Stream ecocytem resposnse to
restoration across urban tributaries of the River Thames, London”

The molecular descriptors that influence gill uptake of pharmaceuticals identified in a recent paper by Elisabeth Chang

The molecular descriptors that influence gill uptake of pharmaceuticals identified in a recent paper by Elisabeth Chang

Launch of the Suffolk Sustainability Institute

Launch of the Suffolk Sustainability Institute – https://www.uos.ac.uk/content/suffolk-sustainability-institute

Sublethal exposure to copper supresses the ability to acclimate to hypoxia in a model fish species.

Fitzgerald, J.A., Urbina, M.G., Rogers, N.J., Bury, N.R., Katsiadaki, I., Wilson, R.W., Santos, E.M. 2019
Sublethal exposure to copper supresses the ability to acclimate to hypoxia in a model fish species.
Aquatic Toxicology 217:105325. doi: 10.1016/j.aquatox.2019.105325.

Biomonitoring of pesticides, pharmaceuticals and illicit drugs in a freshwater invertebrate to estimate toxic or effect pressure.

Miller, T.H., Ng, K.T., Bury, S.T., Bury, S.E., Bury, N.R., Barron, L.P. 2019 Biomonitoring of pesticides,
pharmaceuticals and illicit drugs in a freshwater invertebrate to estimate toxic or effect pressure.
Environment International. 129:595-606. doi: 10.1016/j.envint.2019.04.038

Influence of urban river restoration on nitrogen dynamics at the sediment-water interface.

Lavelle, A.M., Bury, N.R., O'Shea, F.T., Chadwick, M.A. 2019. Influence of urban river restoration on
nitrogen dynamics at the sediment-water interface. PLoS One. 14(3):e0212690. doi:
10.1371/journal.pone.0212690.

Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss).

Bussolaro, D., Wright, S.L., Schnell, S., Schirmer, K., Bury, N.R., Arlt, V.M. 2019. Co-exposure to
polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1)
and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss).
Environmental Pollution. 248:706-714. doi: 10.1016/j.envpol.2019.02.066

The use of molecular descriptors to model pharmaceutical uptake by a fish primary gill cell culture epithelium.

Chang, E.D., Hogstrand, C., Miller, T.H., Owen, S.F., Bury, N.R. 2019. The use of molecular descriptors
to model pharmaceutical uptake by a fish primary gill cell culture epithelium. Environmental Science
and Technology 53, 706-714. doi: 10.1021/acs.est.8b04394

Prediction of bioconcentration factors in fish and invertebrates using machine learning.

Miller, TH; Gallidabino, MD; MacRae, JI; Owen, SF; Bury, NR; Barron, LP. 2019. Prediction of
bioconcentration factors in fish and invertebrates using machine learning. Science of the Total
Environment, 648, 80 – 89. doi: 10.1016/j.scitotenv.2018.08.122

Machine Learning for Environmental Toxicology: A Call for Integration and Innovation.

Miller, TH; Gallidabino, MD; MacRae, JI; Hogstrand, C; Bury, NR; Barron, LP; Snape, JR; Owen, SF.
2018. Machine Learning for Environmental Toxicology: A Call for Integration and Innovation.
Environmental Science and Technology 52, 12953 – 12955. doi: 10.1021/acs.est.8b05382

A review of the pharmaceutical exosome in aquatic fauna.

Miller, T.H. Bury, N.R., Owen, S.F., McRae, J.I., Barron, L.P. 2018. A review of the pharmaceutical
exosome in aquatic fauna. Environmental Pollution 239, 129-146. doi: 10.1016/j.envpol.2018.04.012

Considering aspects of the 3Rs principles within experimental animal biology.

Sneddon, L.U., Halsey, L.G., Bury, N.R. 2017.

Journal for Experimental Biology, 220, 3007 – 3016. doi: 10.1242/jeb.147058

Uptake, biotransformation and elimination of selected pharmaceuticals in a freshwater invertebrate measured using liquid chromatography tandem mass spectrometry.

Miller, T.H., Bury, N.R., Owen, S.F., Barron, L.P. 2017.

Chemosphere, 183, 389-400. doi: 10.1016/j.chemosphere.2017.05.083

Do polyethylene microplastic beads alter the intestinal uptake of Ag in rainbow trout (Oncorhynchus mykiss)? Analysis of the MP vector effect using in vitro gut sacs.

Khan, F.R., Boyle, D., Chang, C., Bury, N.R. 2017.

Environmental Pollution, 231, 200-206.

Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, tetatogenic and genotoxic effects in Danio rerio (zebrafish) embryos

Sogbanmu, T.O., Nagy, E., Phillips, D.H., Arlt, V.M., Otitoloju, A.A., Bury N.R. 2016.

Environmental Science and Pollution Research, 23, 14489-14501

The first attempt at non-linear in silico prediction of sampling rates for polar organic chemical integrative samplers (POCIS)

Miller, T.H., Baz-Lomba, J.A., Harman, C., Reid, M.J., Owen, S.F., Bury, N.R., Thomas, K.V., Barron, L.P. 2016.

Environmental Science and Technology. 50, 7973-7981.

The evolution, structure and function of the ray finned fish (Actinopterygii) glucocorticoid receptors

Bury, N.R. 2017

General and Comparative Endocrinology 

 

Regulation of plasma glucose and sulfate excretion in Pacific Hagfish, Eptatretus stoutii is not mediated by 11-deoxycortisol

Clifford, A.M., Bury, N.R., Schultz, A.G., Ede, J.D., Goss, B.L., Goss, G.G. 2017. Regulation of plasma glucose and sulfate excretion in Pacific Hagfish, Eptatretus stoutii is not mediated by 11-deoxycortisol.

General and Comparative Endocrinology, 247, 107-115.

Work begins on new approach to estimate bioaccumulation of aquatic toxins in fish

July 2017:

The overall objective of our research is to reduce the uncertainty related to the estimation of bioaccumulation of organic chemicals in fish in ecological risk assessment (ERA). Based on a combination of different in vitro approaches to estimate chemical uptake and biotransformation with toxicokinetic (TK) and quantitative structure activity relationship (QSAR) models, we aim to develop a tiered approach, integrating modelling with testing strategies to aid in the rapid assessment of bioaccumulation potential (Figure 1). The tiered testing strategy can provide information and guidance for (1) bioaccumulation (hazard) assessment; (2) refining exposure estimation to support risk assessment; (3) TK models to aid in the interpretation of toxicity testing data and to guide experimental testing; and (4) knowledge as input into adverse outcome pathways (AOPs) to strengthen quantitative links between molecular or cellular events and apical effects.

Principal Investigator Prof. Kristin Schirmer with collaboration from:

  • Prof Christer Hogstrand, Professor of Molecular Ecotoxicology, King’s College London, London, UK
  • Dr Nic Bury, University of Suffolk, UK
  • Prof Helmut Segner, Professor, University of Bern (UoBern), Bern, Switzerland
  • Dr Jon Arnot, President, ARC Arnot Research and Consulting Inc., Toronto, ON, Canada
  • Dr Michelle Embry, Associate Director, Environmental Science, ILSI Health and Environmental Sciences Institute, Washington, USA

Read more…

Targeted metabolomics of Gammarus pulex following controlled exposure to selected pharmaceuticals in water

Gόmes-Canela, C., Miller, T.H., Bury N.R., Tauler, R., Baron, L.B. 2016.

Science of the Total Environment. 562, 777-788.

Presentations at SETAC Europe 27th Annual Meeting

Three posters presented:

  • Elisabeth Chang gave one entitled “Understanding factors that influence ionisable xenobiotic bioaccumulation using a Fish in vitro Gill Cell Culture System (FIGCS)”
  • Dr. Tom Miller gave one on “iNVertox: Rapid intelligent in silico predictions of sub-lethal ecotoxicology effects in invertebrates following pharmaceutical exposure
  • Dr. Jon Arnot presented a poster corner presentation on behalf on the ECO34 team entitled “A tiered testing strategy for rapid estimation of bioaccumulation by a combined modelling in vitro testing approach: identification of candidate test chemicals”

Tom also gave a talk entitled Investigating the application of artificial neural networks to predict the bioconcentration of xenobiotics in fish and invertebrates.

Nic Bury also co-chaired a session with Dr. Eric van Genderen entitled – Interpreting biological effects of metals and their mixtures in the aquatic and terrestrial environment

Dr. Jennifer Fitzgerald joins as a Post-doc on the CEFIC ECO34 grant

Dr. Jennifer Fitzgerald, from Dr Eduardo Santos group at the University of Exeter joins as a Post-doc on the CEFIC ECO34 grant.

BBSRC grant starts entitled – iNVERTOX: Rapid intelligent in silico prediction of sub-lethal ecotoxicological effects in invertebrates following pharmaceutical exposure

April 2017:

Lead PI Dr. Leon Barron (KCL), Post-doctoral researcher Dr. Tom Miller, co-I Dr Nic Bury (University of Suffolk, co-I Dr James MacRae (Crick Institute)

This project will generate groundbreaking knowledge on the subtle effects of pharmaceuticals in the environment on a model freshwater benthic invertebrate, Gammarus pulex. As excellent indicators of surface water quality, these species are consistently impacted by pharmaceuticals and their metabolites at the ng-ug/L level mainly via sewage treatment plant effluents. Non-lethal phenotype-level effects, metabolomics studies and analytical measurements of >60 pharmaceuticals in G. pulex will be combined to generate biologically-inspired artificial neural networks and/or support vector machine models for rapid prediction of ecotoxicity from molecular level changes. In particular, models will be used to (1) predict growth rate, feeding rate, ventilation and locomotion effects; (2) identify metabolic pathways affected by pharmaceuticals; and (3) reduce the number of animals required for ecotoxicity testing in the future. The project will house five work packages (WPs): (1) Bioanalytical methods for G. pulex; (2) Pharmaceutical exposures and non-lethal effect measurement; (3) Metabolomics of exposed G. pulex and pharmaceutical residue measurement in biota; (4) Machine learning methods to model metabolomics/chemical measurement datasets to predict sub-lethal effects and/or affected pathways; and (5) Bioevaluation of novel biomarkers of exposure to pharmaceuticals. Metabolite/chemical analysis will be performed using gas and liquid chromatography coupled to (high resolution) mass spectrometry. Correlations with phenotypic effects will be identified using, for example, principal component analysis, Volcano plots and Z-transformation to rapidly identify dependent biomarkers. Linkage to pharmaceutical exposure will be built-in to models via internal pharmaceutical concentrations. Lastly, and in reverse, the prediction of molecular level changes will be investigated from quantitative structure-activity relationships and phenotype data for biomarker discovery and read-across.

Read more …

Dr Nic Bury moves to the University of Suffolk

University-of-Suffolk_Logo_CMYK
January, 2017:

After 16 years at King’s, I have decided to embark on a new challenge and have taken up a position at the University of Suffolk in Ipswich. The research continues. I still have on-going projects at King’s in collaboration with Prof Christer Hogstrand and Dr. Mike Chadwick and over the coming few years intend to build a research group here in Ipswich. The labs are state-of-art here in Ipswich and I hope myself and my students can enjoy continued collaborations around the globe.

Procedures for the reconstruction, primary culture and experimental use of rainbow trout epithelia.

Schnell, S., Stott, L.C., Hogstrand, C., Wood, C.M., Kelly, S.P., Part, P., Owen, S.F., Bury N.R. 2016. Procedures for the reconstruction, primary culture and experimental use of rainbow trout epithelia.

Nature Protocols 11, 490 – 498.

Assessing the reliability of uptake and elimination kinetics modelling approaches for estimating bioconcentration factors in the freshwater invertebrate, Gammarus pulex

Miller. T.H., McEneff, G.L., Stott, L.C., Owen, S.F., Bury, N.R. Barron, L.P. 2016. Assessing the reliability of uptake and elimination kinetics modelling approaches for estimating bioconcentration factors in the freshwater invertebrate, Gammarus pulex.

Science of the Total Environment – 547, 396-404.

Hypoxia Suppressed Copper Toxicity during Early Development in Zebrafish Embryos

Fitzgerald J.A., Jameson H.M., Dewar Fowler, V.E., Bond, G.L., Bickley L.K., Uren Webster, T.M., Bury, N.R., Wilson, R.J., Santos, E.M. Hypoxia Suppressed Copper Toxicity during Early Development in Zebrafish Embryos in a Process Mediated by the Activation of the HIF Signaling Pathway.

Environmental Science & Technology, 50, 4502-4512.

Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, tetatogenic and genotoxic effects in Danio rerio (zebrafoish) embryos

Sogbanmu, T.O., Nagy, E., Phillips, D.H., Arlt, V.M., Otitoloju, A.A., Bury N.R. 2016. Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, tetatogenic and genotoxic effects in Danio rerio (zebrafoish) embryos.

Environmental Science and Pollution Research.

 

Targeted metabolomics of Gammarus pulex following controlled exposure to selected pharmaceuticals in water

Gόmes-Canela, C., Miller, T.H., Bury N.R., Tauler, R., Baron, L.B. 2016. Targeted metabolomics of Gammarus pulex following controlled exposure to selected pharmaceuticals in water.

Science of the Total Environment.

SETAC Europe 26th Nantes 22nd – 26th May 2016

Tom Miller, current PhD student with Dr. Leon Barron, gave two excellent talks on his recent work assessing pharmaceutical uptake in Gammarus pulex Bioconcentration and biotransformation of selected pharmaceuticals in the freshwater amphipod, Gammarus pulex and the use of POCIS to monitor water contaminants (In silico prediction of sampling rates for polar organic chemical integrative samplers (POCIS)).

Nic Bury also chaired a session on Metal Mixtures (Interpreting Biological Effects of Metals and Their Mixtures: Chairs Eric J. Van Genderen, Nicolas Bury).

Methods for culturing FIGCS published in Nature Protocols

New CEFIC-LRI Grant (€ 500,000) with Prof. Kristin Schirmer (EAWAG) (Lead PI), Prof Helmut Segner (University of Bern), Dr Jon Arnot (Arnot Research and Consulting Inc) and Dr Michelle Embury (ILSI) entitled A tiered testing strategy for rapid estimation of biaccumualtion by a combined modelling – in vitro test approach.

It is acknowledged that biotransformation is an uncertainty when estimating the bioaccumulation of organic chemicals. The aim of the current project is to develop in vitro methods, that include models of the gill, gut and liver as well as cell lines, as a rapid assessment for uptake and biotransformation pathways of chemicals.

For more details see http://cefic-lri.org/projects/eco34-a-tiered-testing-strategy-for-rapid-estimation-of-bioaccumulation-by-a-combined-modelling-in-vitro-testing-approach/

Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS).

Schnell, S., Bawa-Allah, K., Otitoloju, A., Hogstrand, C., Miller, T.H., Barron, L.P. Bury, N.R. 2015. Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS). Ecotoxicology and Environmental Safety 120, 279-285.

http://www.ncbi.nlm.nih.gov/pubmed/26093110

A primary fish gill cell culture model to assess pharmaceutical uptake and efflux: Evidence for passive and facilitated transport.

Stott LC, Schnell S, Hogstrand C, Owen SF, Bury NR. 2015 A primary fish gill cell culture model to assess pharmaceutical uptake and efflux: Evidence for passive and facilitated transport. Aquat Toxicol. 159:127-37.

http://www.ncbi.nlm.nih.gov/pubmed/25544062

Influence of polyethylene microplastic beads on the uptake and localization of silver in zebrafish (Danio rerio)

Khan, F.R., Syberg, K., Shashoua, Y., Bury N.R. 2015. Influence of polyethylene microplastic beads on the uptake and localization of silver in zebrafish (Danio rerio). Environmental Pollution 206, 73-79.

http://www.ncbi.nlm.nih.gov/pubmed/26142753

Influence of chloride on silver bioavailability and toxicity in salmonids.

Bury, N.R., and C. Hogstrand. 2002. Influence of chloride on silver bioavailability and toxicity in salmonids. Environmental Science and Technology 36: 2884-2888.

http://www.ncbi.nlm.nih.gov/pubmed/12144263

Physiological impact of salinity increase at organism and red blood cell levels in the European Flounder.

Jensen, F.B.., Lecklin, T., Busk, M., Bury, N.R., Wilson, R.W., Wood, C.M., and M. Grosell. 2002 Physiological impact of salinity increase at organism and red blood cell levels in the European Flounder. Journal of Experimental Marine Biology and Ecology 274: 159-174.

http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=15&SID=T2xXoIu6uJwzf1p3pNp&page=1&doc=2